WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

STUDY OF PREVALENCE SOME PATHOGENICITY-ASSOCIATED VIRULENCE FACTOR GENES IN UROPATHOGENIC E.COLI ISOLATES AND ABILITY OF TRANSFERRING THEM TO OTHER RELATED AND NON-RELATED SPECIES IN AL NAJAF PROVINCEIRAQ

Ahmed Ali Al-Tufaili*, Dr. Falah Salim Manhal, Dr. Ahlam Kadhem Naeem

ABSTRACT

Background: The generally accepted hypothesis today is that UPEC involved from nonpathogenic strains by acquiring new virulence factor from accessory DNA horizontal transfer located at the chromosome or plasmid level. Aim of study: investigate the genetic determination of some pathogenicity-associated virulence factors (PAVFs) genes such as fimH, hlyA and iucC genes in UPEC and capability of transferring of them from UPEC to related and non related species such as E.coli JM 109 and P. aeruginosa as well as evaluation of genes transferring effeciency. Method: During the period from May 2014 to November 2014.a total of 290 samples has been collected from patients suffering from Urinary tract infections (170 samples) and burn infections (120 samples) from Alzahraa and Alsadr teaching Hospital as well as private clinics analytical laboratories and Central Health Laboratory in Al-Najaf Al- Ashraf City Results: seventy (41.2%) of isolates were belong to Uropathogenic E.coli whereas out of 120 burn infection samples only 60(50%) isolates were belong to P.aeruginosa depending on identification by microscopic, morphologic, genetic and biochemical tests. So fimH, hlyA and iucC revealed that 48(69%), 6(9%) and 56(80%) isolates were possess these gene respectively, while 12(17%) isolates showed negative results (absence of any target genes) , to confirm ability of these gene to transferred from local UPEC isolates to E.coli JM 109 as well as to local cured P.aeruginosa that selected from conjugation and transformation possess, results of that showed fimH had ability to transfer by conjugation and transformation to E.coli JM 109 while it was unable to transferred to cured P.aeruginosa isolated by these two methods. As well as hlyA gene had ability to transfer to cured P.aeruginosa by transformation process while unable to transfer by conjugation as well as it had no ability to transfer by both methods to E.coli JM 109. results of conjugation and transformation showed inability of iucC to transfer by two methods to E.coli JM 109 and cured P.aeruginosa, so transformation efficiency was 55.75 times greater than conjugation efficiency and conjugation efficiency between UPEC strain and P.aeruginosa with regard to hlyA gene transferability was 1.75 times greater than the transformation efficiency. Conclusion: Genetic transformation efficiency between UPEC strain and E.coli JM109 with regard to fimH gene transferability is greater than the conjugation efficiency, whilst conjugation efficiency between UPEC strain and P.aeruginosa with regard to hlyA gene transferability was greater than the transformation efficiency.

Keywords: PAVFs, virulence factor, UPEC, fimH, hlyA and iucC genes, horizontal gene transferring.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More