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Abstract

EFFECT OF DENSITY GRADIENT TECHNIQUE ON CRYOPRESERVED SEMEN OF ASTHENOZOOSPERMIC MEN USING PENTOXIFYLLINE

Prof. Dr. Saad AL-Dujialy*, Assist. Prof. Dr. Mohammad Oda Salman, Sura Badie AL-Naqeeb

ABSTRACT

Background: Pentoxifylline (PTX) as motility stimulant have shown positive effect on in vitro sperm activation and improved the forward movement. Indication for sperm cryopreservation is expanding results frozen sperm being as good as fresh sperm in fertilizing oocytes. Objective: This experiment has been designed to examine the certain sperm function parameters after in vitro preparation of cryopreserved semen by PTX using density gradient method. Materials and methods: forty five men were involved in this study that divided into two groups, namely; asthenozoospermic group(no=25), and normozoospermic group (no=20), depending on the results of semen analysis. Ejaculated semen were analyzed and in vitro activation technique after cryopreservation-thawing was adopted by using Ham’s F-12 (control media) and PTX medium to evaluate the sperm functional characteristics before and after cryopreservation and after activation of cryopreserved human semen by density gradient technique. Results: in vitro activation of cryopreserved semen with PTX medium have shown a positive effect on sperm motility, grade activity of progressive forward movement and morphologically normal sperm (MNS) in control and treated group compared to post-thawing and other media. Conclusion: The study has shown that adding of PTX medium (1 ml) to the cryopreserved semen samples resulted in an improvement in certain sperm functional parameters. It is recommended for more studies in future to carrying out the effect of these stimulants on the DNA of cryopreserved semen.

Keywords: Pentoxifylline, In vitro activation, cryopreservation, asthenozoospermia.


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