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Abstract

EFFECT OF FRACTION 2 OF PORTULACA OLERACEA ON PLASMA GONADOTROPHINS AND TESTOSTERONE LEVELS AND THEIR RECOVERIES IN MALE WISTAR RATS

Oyedeji K. O.*, Oladosu I. A. and Adegoke Olajire

ABSTRACT

Objective: To investigate effect of chromatographic fraction 2 of Portulaca oleracea on plasma gonadotrophins and testosterone levels and their recoveries in male rats. Methods: Air-dried specimen of Portulaca oleracea was cold-extracted in methanol for 72 hours. The resulting methanol extract was then subjected to open column chromatography on silica gel for fractionation. Out of 5 fractions obtained, fraction 2 was then subjected to male rats’ hormonal bioassays. Twenty male rats (120-150 g) were divided into control and fraction 2(1, 2, 3 mg/kg) treated groups (5 per group) for hormonal assays study. The animals were orally treated on daily basis for 50 days and allowed a recovery (withdrawal) period of 50 days after which hormonal assays were carried out at the end of dosing and recovery periods. Plasma LH, FSH and testosterone levels were assayed using Enzyme – Linked Immunosorbent Assay (ELISA) technique. Results: Treatment of rats for 50 days with fraction 2(2 mg/kg, 3 mg/kg) caused significant (p<0.05) increase in LH levels relative to control. Fraction 2(1 mg/kg, 3 mg/kg) caused significant (p<0.05) increase in FSH levels relative to control. Fraction 2(1 mg/kg, 3 mg/kg) caused significant (p<0.05) decrease in testosterone levels relative to control. There was a significant (p<0.05) decrease in LH level of fraction 2(3 mg/kg) recovery group relative to control. Conclusion: It can therefore be concluded that fraction 2 of Portulaca oleracea probably induced significant changes on plasma gonadotrophins and testosterone levels through the hypothalamic – pituitary – testicular axis which could result in sterility.

Keywords: Fraction 2, Testosterone, Luteinizing hormone (LH), Follicle stimulating hormone (FSH), Rats.


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