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Abstract

EVALUTION OF ANTIBACTERIAL ACTIVITY OF ADATHODA VASICA LEAF EXTRACT

E. Shravya Puri* and A. Sabitha Rani

ABSTRACT

The present study was undertaken to evaluate the antimicrobial activity of petroleum ether leaf extracts of Adathoda vasica against selected pathogens. Antimicrobial activity of this plant was assessed against some pathogens Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, E. coli. Antibacterial activity was determined by using agar cup bioassay method. Petroleum ether extract was found to be effective against tested bacteria. Antimicrobial activity was measured at different concentrations. The present study showed significant antibacterial activity of leaf extracts of Adathoda vasica against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, E.coli. 200mg/ml concentration of petroleum ether extract of Adathoda vasica showed the maximum inhibition against Pseudomonas aeruginosa 1.8 cm followed by Staphylococcus aureus 1.2cm, Bacillus subtilis 1.1cm, E.coli 0.9cm. The present study revealed the presence of antibacterial activity of Adathoda vasica against selected pathogens. Studies on the minimum inhibitory concentration and minimum bacterial concentrations were demonstrated against Bacillus subtilis 1.1cm, E.coli 0.9cm. and the highest minimum inhibitory concentration was exhibited against Pseudomonas aeruginosa 1.8 cm, Staphylococcus aureus 1.2cm, among bacteria E.coli showed lowest minimum inhibitory concentrations.

Keywords: Adathoda vasica, petroleum eather, antibacterial activity.


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