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Abstract

IN VITRO ALPHA AMYLASE INHIBITORY ACTIVITY OF ETHANOL AND HOT WATER EXTRACT OF POLYHERBAL FORMULATION

*Ajay Mandal and P. Jagan Mohan Reddy

ABSTRACT

In vitro α-Amylase inhibitory activity of polyherbal preparation, a combination of six selected medicinal plants. The antidiabetic potential of the aqueous extract and ethanolic extract of Ocimum sanctum, Withania somnifera, Gymnema sylvestre, Tinospora cordifolia, Phyllanthus emblica and Coleus forskohlii. Diabetes has become a common global health problem that affects >170million people worldwide. It is one of the leading causes of death and disability. It is estimated that by 2030, the number will rise to 366 million (www.who.int). The majority of diabetes (~90%) is type 2 diabetes (T2D) caused by a combination of impaired insulin secretion from pancreatic beta cells and insulin resistance of the peripheral target tissues, especially muscle and liver. Traditional Medicines obtained from medicinal plants are used by about 40-60% of the world’s population. Though there are many approaches to control diabetes and its secondary complications, herbal formulations are preferred due to lesser side effects and low cost. In the present study focused on screening of ethanolic and hot water extract of polyherbal formulation for antidiabetic activity with respect to enzymatic assay as such and α- amylase inhibition assay. Polyherbal formulation (PHF) extracts of two different organic solvents exhibited significant reduction in alpha-amylase activity. Acarbose standard antidiabetic drug which was used as positive control at concentrations 10-100μg/ml showed PPA inhibitory activity from 18.75% to 58.60% with an IC50 value of 83.19μg/ml [Table-17 and Graph-15].PHF of hot water extract at concentration10-100μg/ml showed maximum inhibitory effects on Alpha-Amylase activity from 23.44% to 88.22% with an IC50 value of 41.84μg/ml respectively [Table-18 and Graph-16]. PHF of Ethanolic extracts at concentrations 10-100μg/ml showed moderate inhibitory effects on Alpha-Amylase activity from 17.05% to 70.05% respectively with an IC50 value of 66.62μg/ml. [Table-19 and Graph-17]. Out of two solvent selected the hot water extract solvent showed maximal inhibition indicates its maximal therapeutic efficacy.

Keywords: Polyherbal formulation (PHF), Phytochemicals, Alpha-Amylase inhibition and Acarbose.


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