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Abstract

CAFFIEC ACID FROM THE LEAVES IN TECOMA STANS: QUANTIFCATION AND VALIDATION BY USING HPLC

Sushmitha, Naira Nayeem*

Krupanidhi College of Pharmacy, Bangalore-35, India.

ABSTRACT

The aim of current study was to develop and validate a simple, reliable and fast analytical procedure for the determination of caffiec acid in Tecoma stans using high-pressure liquid chromatography (HPLC).Reverse phase Phenomenex (US) C18 Column 250 x 4.6mm I.D, 5μ Particle Size was used for the development. The mobile phase was prepared separately with 0.1N Potassium dihydrogen Phosphate buffer (mobile phase A) and methanol (mobile phase B). The pH of the mobile phase (A) was maintained at 5.2 with prior correction with 10% phosphoric acid. The flow rate was maintained at 1.0 ml/min with an injectable volume of 20 micro l. The method was validated for linearity accuracy and robustness. The retention time for caffiec acid was found to be 2.915. The method produced linear responses in the concentration range of 50-150%. The amount of caffiec acid was found to be 4.9mg/gm. Hence a rapid, simple, accurate and specific HPLC method was validated for quantitative estimation of caffiec acid present in the dried leaves of Tecoma stans.

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