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Abstract

EFFECT OF EXTENDERS AND PRESERVATION PERIODS IN SOME SEMEN CHARACTERISTICS OF AWASSI RAMS

Nameer M.H. Albiaty*, Hazem J.K. Alobaidi, Abbas F. Kareem, Ali M. Al-Hakim, Anmar Y. Alnaeb and Alkhazraji, A.A.H.

ABSTRACT

A study was conducted to investigate the effect of the different extenders and preservation periods in some characteristics of Awassi ram semen. Semen from three Awassi rams was collected weekly then preserved for three days in refrigerator after diluted with three different extenders (Tris, hydroxymethyl aminoethane; Sodium citrate; and Milk). Diluted semen was evaluated daily by means of five traits. Significant differences (p < 0.05) between extenders were found in progressive motility and acrosome defects, while for other characteristics (pH, dead and abnormal sperm percentages) were not significant. Tris and sodium citrate-based extenders showed superiority (P<0.05) in comparison with milk - based extender in progressive motility (63.87 and 61.25% vs. 39.12% respectively), in the contrast, acrosome defect values were (8.95 and 10.15% vs. 11.47% respectively). Preparation processes and storage periods showed significant (p < 0.05) effect on all traits except dead sperm percentage. It was concluded that tris and sodium citrate extenders were preferable on milk Extender. Also, preparation processes and storage periods at 5˚C affected semen quality. The optimum preservation period for Awassi ram semen was not more than two days.

Keywords: Awassi ram, extenders, Semen preservation.


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