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Abstract

ALTERNATIVE TO LABORATORY ANIMALS FOR ISOLATED TISSUE EXPERIMENTATION USING CHICKEN ILEUM PREPARATION

Marwan Saedi*

ABSTRACT

For the teaching purposes to use isolated strip preparations from various organs, the laboratory animal species has to be sacrificed just for a piece of tissue. Pharmacology as a subject depends largely on experiments conducted in laboratory animals. Experimental animals like rat, guinea pig, rabbit, etc. are used for the biological assay. The present study was aimed to develop ex vivo model for pharmacological experimentation, which will mimic the actual laboratory condition without sacrificing the experimental animals. Dose response curve of acetylcholine is carried out in different conditions by changing different parameters and compare the result. The effect of acetylcholine in terms of its type of agonist was predicted based on kymograph and curve had been obtained. The chicken ileum, duodenum and rat ileum were also evaluated for four- and three-point bioassay, respectively. The results suggested that acetylcholine produced a dosedependent increase in contraction in both chicken and rat ileum preparation. The concentration response curve of acetylcholine in chicken ileum shows good response by changing the parameters other than that used in rat ileum. It is concluded that isolated chicken ileum and duodenum preparation can be employed for routine experiments of pharmacology subject and the use of these isolated preparations is a novel approach for managing pharmacological experiments and importantly, without sacrificing the experimental animals.

Keywords: Acetylcholine, PSS, chicken ileum, four point bioassay, three point bioassay.


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