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Abstract

ISOLATION AND ESTIMATION OF SERRATIOPEPTIDASE AND PROPHENOLOXIDASE ENZYME ACTIVITY IN DIFFERENT RACES OF SILKWORM BOMBYX MORI L.

Mamatha C.*, Bela Zutshi and Jayaprakash

ABSTRACT

Extraction and estimation of serratiopeptidase and prophenoloxidase enzymes from five different races of silkworm Bombyx mori L. [Bivoltine (PM, NK2), Multivoltine (CSR2, MH1) and Multi-hybrid (RP9×NK2)] has been carried out. Serratiopeptidase activity was analysed by casein hydrolysis method since it is a protease enzyme, while prophenoloxidase by DNS method and total protein content by Lowry’s method. It is found that serratiopeptidase and prophenoloxidase production was more in multi-hybrid (RP9×NK2) compared with those of bivoltine and multivoltine breeds.

Keywords: Bivoltine, multivoltine, multi-hybrid, serratiopeptidase, prophenoloxidase.


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