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S.Afroz Begum, C. Gopinath*, N. Pramod, M. NagendraBabu, N.C. SreeVaishnavi, S. Chand Basha


Various substituted formazan derivatives have received considerable
importance during last decade as they are covered with wide variety of
biological and pharmacological activities and have a wide range of
therapeutic importance. Based on this a series of new heterocyclic
containing formazan derivatives are synthesized by the treatment of
pyridine-4–carbaldehyde with phenylhydrazine in presence of natural
acidic catalyst like lemon juice yields Schiff bases. This Schiff base is
then treated with different diazonium salts in presence of pyridine to
form a novel series of Formazan derivatives. The synthesized
compounds were characterized by physical studies like solubility,
melting point, TLC and subjected to spectral studies like IR, 1H-NMR
spectroscopy and Mass spectrometry. All the synthesized compounds
were screened for biological activity like In-vitro anti-tubercular activity was performed by
MicroplateAlamar Blue Assay method (MABA) by using Pyrazinamide, Ciprofloxcin and
Streptomycin as reference standards. Results suggested that electron withdrawing groups like
nitro, chloro, containing compounds shown potent anti-tubercular activity. Rest of
compounds showed mild to moderate activity.

Keywords: Formazans, Diazotization, Antitubercular activity.

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