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Abstract

BIOCHEMICAL MARKER ANALYSIS IN DIABETIC PATIENTS

Samer Salim Abed, Fadhil Hamad Zaidan, Balasubramanian Sathyamurthy*

ABSTRACT

Diabetes mellitus is a common disease of the world. Diabetes is a metabolic disorder where insulin is not produced or used in the human body. Insulin hormone is required to convert sugar, starches, and other food into energy. Diabetes mellitus is a disorder which is characterized by high levels of blood glucose (sugar). In human system, the blood glucose levels are regulated in a very narrow range, which is done with insulin and glucagon. The function of glucagon regulates the release of glucose in liver from its cells into the blood, for the production of energy. Biochemical markers are important in determining the disease status. However the data related to the correlation between different markers like fasting and post prandial glucose levels, HbA1c level and Vitamin D3 level with different age group is inadequate. In our study about 10 patients of different age groups were selected as experimental materials. The study was intended to mainly study the blood markers which can help in detecting the diabetic status of the people with informed consent. The markers of interest were Glucose levels both in fasting and post prandial, HbA1c levels in blood samples, and Vitamin D3 levels. All these are found to be positively correlated with different age groups. The levels of Glucose both in Fasting and post prandial state are estimated by spectrophotometry. The levels of vitamin D3 and their correlation among different age groups in serum samples were also studied. The HbA1C values in the serum were quantitatively estimated by Turbidimetric Inhibition Immunoassay (TINA, Tina-quant HbA1c II). The results obtained were found to be significant especially in the age group 56-60 and 61-65 age groups. All the results were confirmed and significance was given by the student’s t test. (p<0.05). The vitamin D3 levels are inversely correlated to the age groups. This may be a finding that, the Vitamin D3 deficiency may be one of the causes of diabetes.

Keywords: The function of glucagon regulates the release of glucose in liver from its cells into the blood, for the production of energy.


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