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            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
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Abstract

IN SILICO MODELING AND ANALYSIS OF THE LARGEST HEMOLYSIN PROTEIN OF STAPHYLOCOCCUS HAEMOLYTICUS

*Bakr M. AL-Alway and Zahra M. AL-Khafaji

ABSTRACT

Hemolysin protein is cytolytic toxin produced by Staphylococcus haemolyticus that effects the human erythrocytes and has nonspecific membrane toxicity to other mammalian cells. It is worth to be inactivated as a virulence factor for bacterial treatment. For the prediction of 3D-Structure of this protein several online modeling and prediction servers were used, the outputs of some servers had shown a poor quality and prediction but, Raptor X server output showed some promising result as it had 97% query coverage. The model was validated by QMEAN score, ProSA server and refined by 3D Refine. Finally the model was revalidated using ProAS server. Significant differences were observed after refinement in the quality of the model according to ProSA server.

Keywords: Staphylococcus haemolyticus, In Silico, Homology Modeling, Raptor X, University of Baghdad.


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