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Dr. Muhammad Baqir MR Fakhrildin
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Abstract

EFFECT OF GAMMA AND BETA RADIATION ON MULTI –DRUG RESISTANT(MDR) PSEUDOMONAS AERUGINOSA RESISTANCE COLISTIN ISOLATED FROM SKIN, BURNS, WOUND INFECTIONS AND INHIBITION OF MEXXY, MEXAB-OPRM EFFLUX PUMPS

Nebras R. Mohammed*, Hanna S. Sabaa, Mohammed F. Al-Marjani and
Khalid H. Mahdi

ABSTRACT

It was obtained P.aeruginosa, it has been activated then held conducted her dilution according to the method used below, it has been exposed to Beta and Gamma irradiation that emitted from 137CS source with activity 1.712 𝛍ci, 5 𝛍ci, 9 𝛍ci and 60Co source with activity 1 𝛍ci and 22Na source with activity 1 𝛍ci, in different dose calculated of KGy which emitte Beta and Gamma irradiation. for a time of (2,3,) hours for a contenouse exposure, it has been calculated number of colony and percentage of killing of P.aeruginosa resistance Colistin. It has been obtained to increase the times increased the percentage of killing of P.aeruginosa, the morphology of colony were different from origin appear her clear change Detection of Gene expression of P.aerginosa resistance Colistin before exposure to Beta and Gamma irradiation and after exposure to Beta and Gamma irradiation was performed done by using q RT-PCR technique after RNA extraction of P.aerginosa and cDNA synthesis, calculated gene expression according to Livak equation to detection of gene expression of MexXY and MexAB-OprM efflux pumps by studied mexB and mexX gene, The results showed that gene expression were high to MexXY and MexABOprM efflux pumps of P.aerginosa resistance Colistin before exposure to Beta and Gamma irradiation. but The results showe gene expression were very fewer to MexXY and MexABOprM efflux pumps of P.aerginosa after exposure to Beta and Gamma irradiation, the gene expression of MexXY, MexAB-OprM efflux pumps reach zero. The Beta and Gamma irradiation was efficiency inhibitors to MexXY and MexAB-OprM efflux pumps. and efficiency to killing P.aeruginosa resistance Colistin. This result give indicat to DNA was effected by Beta and Gamma irradiation and not occur gene expression of efflux pumps.

Keywords: P.aeruginosa, Gamma and Beta irradiation.


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