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Abstract

EVALUATION OF ANTIOXIDANT POTENTIAL AND PHYTOCHEMICAL STUDIES OF ONONIS ANGUSTISSIMA L. (FABACEAE)

Sofiane Guettaf, Nacira Abidli*, Sihem Kariche, Leila Bellebcir and Hamama Bouriche

ABSTRACT

Medicinal plants would be the best source to obtain variety of antioxidant compounds. Effectively, several studies have demonstrated the potential of plant products as antioxidants against various diseases. By their resistance to several stress factors and under extreme climatic conditions, Saharan plants could contain biomolecules potentially useful as antioxidants. Ononis angustissima is an endemic plant of the north of Algerian Sahara. The aerial part were used by local people for its hemostatic properties. According to literature, Ononis angustissima was previously little chemically studied. The originality of the present study consists in scrutinizing the phytochemical composition and evaluating the antioxidant activity of aerial parts of aqueous extract of Ononis Angustissima (AEO) in conditions corresponding to its traditional use. Phytochemical screening was realized to scrutinize the qualitative composition of (AEO) using precipitation and coloration reactions. The total phenolics, flavonoids, tannins and β carotene contents were determined by using spectrophotometric methods. Finally, AEO was submitted to antioxidant activities assays using 3 methods : DPPH, reducing power and β carotene bleaching tests. The obtained results show the presence of different metabolites in AEO such as, phénolics compounds, flavonoids, tannins, terpenoids, glycosides, steroids and saponins. In addition, the quantitative analysis shows a considerable total phenolics, tannins and flavonoids contents. In sum, it was found that EAO possess a good antioxidant activity against bleaching of β carotene, which sustains its use by traditional healers. Its good antioxidant activity may be explained by the high occurence of particullary tannins. Thus, Ononis Angustissima could be candidate for a good source of antioxidant compounds.

Keywords: phytochemical screening, antioxidant activity, DPPH test, reducing power, ? carotene, flavonoids, tannins.


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