Abstract

GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM POMEGRANATE (Punica granatum) LEAF, FLOWER AND FRUIT EXTRACT

B. Vennila* and Dr. S. Anita Kannagi

ABSTRACT

Nanoparticles are one of the drug delivery tools. They can be used either as drug carriers or as the treatment itself. Using this delivery technique, nanoparticles will minimize the side effects of painful chemical therapy as in cancer cases. Several methods have been used to form nanoparticles. One of the simplest ways to form nanoparticles is the chemical reduction reaction in aqueous conditions. It is a chemical reaction that occurs between two components. One of them is the nanoparticles precursor, while the other acts as reducing agent. Several researchers change the chemical reagent with natural products that are enriched with reducing agents compounds such as strawberry, cranberry and others. Using this method, which known as green synthesis of nanoparticle, will produce nanoparticles with less toxicity. The reduction of pure Ag++ ion monitored by the absorption maxima was scanned by UVSpectral photometer at the wave length of 200- 800nm. The sharp bands of silver nanoparticles were observed around 350 and 400 nm in leaf, 450 and 490 nm in flower and fruit. The antibacterial assays against Gram positive (Staphylococcus aureus) and Gram negative strains (pseudomonas) and two pathogenic fungi namely Candida albicans and Aspergillus were also performed by standard disc diffusion method.

Keywords: silver nanoparticle, pomegranate leaf, flower and its fruit extract, silver nitrate biosynthesis.