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Abstract

QUANTITATIVE AND IN VITRO ANTIOXIDANT POTENTIAL OF VARIOUS FRACTIONS OF HELICTERES ISORA ROOTS

Urmila Chaudhary, Veena Sharma* and Shatruhan Sharma

ABSTRACT

Objects: The main object of this study is to explore the antioxidant and phyto-chemistry of various sequential (petroleum ether, benzene, chloroform, ethyl acetate, ethanol and aqueous) extracts of H. isora roots which is a medicinal plant. Methods: In vitro Phytochemical (phenol, flavonoid, proanthocynidins, tannins and saponin) of H. isora roots were estimated and various antioxidant (DPPH, FRAP, total antioxidant activity, metal chelating, hydroxyl radical, superoxide anion, nitric-oxide radical scavenging activity and reducing power assay) assays were performed. Results: The aqueous extract of H. isora exerted significant (P<0.05 and P<0.001) phytochemicals, and was found to have good radical scavenging activity as compared to the others extracts as well as synthetic antioxidants. These results were also supported by IC50 values. Conclusions: From the results of the present investigation, it could be concluded that H. isora roots extracts can be explored as a potential source for the isolation of natural antioxidant compounds.

Keywords: Helicteres isora; Reducing power; Antioxidant activity; Flavonoids; IC50.


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