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Abstract

STUDY ON THE EFFECT OF PARTIALLY PURIFIED RUSSELL’S VIPER (EASTERN REGION, INDIA) VENOM ON HUMAN SPERM

Rashmi Rajappa*, Magesh S Bojan, Reshma.P, Jessen George

ABSTRACT

The specific aim of the present study is to investigate the in vitro effect of partially purified Russell’s viper venom (Eastern region of India) on human sperm motility and viability. Russell’s viper venom was subjected to gel filtration chromatography on Sephadex G-75 column and resolved peaks were pooled and subjected to caseinolytic, hemolytic and trypsin inhibitor activity. Sperms were collected from healthy donors and checked for advanced motility and samples less than 60% motility was not used for study. The sperms were incubated in vitro in phosphate buffer, pH 7.0 containing various concentrations of venom 0-80 mg at 37ºC for different time intervals between 0-60 minutes. Peak I showed caseinolytic, peak II showed indirect hemolytic and peak III protein devoid of trypsin inhibitor activity. Further, the results showed that, the percentages of sperm motility were decreased and the percentages of dead sperms were increased in a time and concentration-dependent manner. Our results revealed that Russell’s viper venom induced a concentration and time-dependent action on sperms motility and viability, which may be due to the alteration in spermatozoa plasma membrane and mitochondrial dysfunction.

Keywords: Russell’s viper venom, sperm, motility.


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