Abstract

COMPARATIVE STUDIES ON CYTOTOXIC, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY AMONG DIFFERENT EXTRACTS OF SEED AND STEM OF Anethum sowa L. AVAILABLE IN BANGLADESH

Muhammad Abdullah Al-Mansur*, Koushik Saha, Nasim Sultana, M. Mahboob Ali Siddiqi

ABSTRACT

The ethyl acetate, dichloromethane, n-hexane, aqueous, methanol extract from seed and stem of Anethum sowa Linn. (Family- Apiaceae) were subjected to antimicrobial screening, brine shrimp lethality bioassay and antioxidant activity test carried out by Free Radical Scavenging Activity (FRST) and Total Phenol Content(TPC). In case of antimicrobial screening, the mild to moderate zone of inhibition was exhibited by n-hexane and dichloromethane extracts of stem than ethyl acetate and methanol extracts while ethyl acetate extract of seed showed very poor activity. Moreover from the results of the brine shrimp lethality bioassay it can be well predicted that methanol extracts of seed and stem demonstrated quite potent activity but methanol extract of seed of A. sowa with LC50 of 7.89±0.21 μg/ml showed more potent activity than other extracts. Besides this, the crude dichloromethane extract of seed showed potent antioxidant activity with IC50 7.44±0.93 μg/ml while the ethyl acetate and methanol extract showed moderate antioxidant activity. In addition, the highest phenolic content was found in dichloromethane extract of seed (90.03±1.09 mg of GAE (gallic acid equivalent) / gm of dried extract) and in case of stem, methanol extract showed highest activity (78.91±1.06 mg of GAE (gallic acid equivalent) / gm of dried extract).

Keywords: Cytotoxicity, Antibacterial activity, Free radical scavenging, Total phenolic content, Anethum sowa.