WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

MARKER BASED STANDARDIZATION OF FORMULATIONS CONTAINING ASHWAGANDHA USING WITHAFERIN A BY HPLC

Dr Pratima A. Tatke* , Dr. Supriya S. Jirge and Dr. Satishchandra Y. Gabhe

ABSTRACT

A high performance liquid chromatography (HPLC) method for the determination of Withaferin A in Withania somnifera formulations was developed. The developed and validated method was successfully applied to analyze the content of Withaferin A in different marketed formulations containing Ashwagandha. The method comprised of a simple isocratic system with reserve phase (RP)-18 column, acetonitrile and water as mobile phase and Withaferin A was detailed at 215 nm. The optimized mobile phase enabled the efficient separation of various components present in formulations within a 10 min analysis, with a retention time of 6.3 minutes for Withaferin A. The method was validated as per International conference of harmonization (ICH) guidelines with a view to demonstrate its selectivity, linearity, precision, accuracy and robustness. The linearity range was 100-1000 ng mL−1and the limit of detection (LOD) and limit of quantification (LOQ) were found to be 30 and 90 ng respectively. The accuracy assay performed revealed a recovery of 96.43-106.57 % from the various marketed formulations analyzed. The inter-day and intra-day reproducibilities were found to have an R.S.D. less than 0.1 %, indicating very good precision.

Keywords:


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More