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Abstract

PRELIMINARY PHYTOCHEMICAL SCREENING AND EFFECT OF AEGLE MARMELOS EXTRACTS AGAINST BACTERIAL COLD WATER DISEASE CAUSING ORGANISM

*Kumar Prasanna and Devaraj Raghunathan

ABSTRACT

Bacterial cold water disease is common in the fresh water streams, ponds, reservoirs and commercial farming ponds which are caused by Flavobaterium psychrophilum of family Flavobacteriaceae. Increasing in sight of antibiotic resistance strains of the fish pathogen the effect of newly developed chemical constituents are required to prevent the infection or disease it causes. The Indian system of utilizing medicinal plants for treating the pathogen with sacred tree of Indian temples Vilvam bark Aegle marmelos is effectively studied for the effectiveness over Flavobacterium psychrophilum. The standard well diffusion method was followed with varying concentration from 50- 250 mg/ml and standard protocol of Harbone’s phytochemical analysis to carry out phytochemical studies. It has shown sensitivity to the bark extracts of hexane and aqueous 15mm each extract at 250mg/ml. The screening of secondary metabolites revealed flavonid, steroid and terpenoids which has made Vilvam bark as a good source of antibacterial agent. Aegle marmelos could be used to treat or prevent the infection related to bacterial cold water disease and higher specification on effective studies need to carry for development of new chemical constituent or drug.

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