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Abstract

IMMOBILIZATION OF ADHATODA VASICA LEAF CELLS FOR ENHANCED PRODUCTION OF VASICINE

Chauhan Pradeep*, Panigrahi Mahendra

ABSTRACT

Traditional techniques of callus and suspension culture do not fully explore the range possibilities of cultured cells. Immobilization is the advanced novel biotechnological approach, through which it is possible to achieve a physiological state conducive for metabolite production that may not have been attended otherwise. Vasicine is the major alkaloid found in Adhatoda vasica, has been used for the treatment of various diseases and disorders particularly of respiratory tract. Leaf cell homogenate was immobilized in calcium alginate and the effect of immobilization on cell growth, cell viability and cells biosynthetic activity was observed.

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