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Abstract

HUMAN PAPILLOMA VIRUS GENOTYPING IN A GROUP OF IRAQI WOMEN BY USING REPRODUCIBLE DNA EXTRACTION FROM FORMALIN FIXED PARAFFIN EMBEDDED SECTIONS

*Anfal Mohammed Al –Taiee, Bsc, Msc., Sanaa Mohammed Alizi BSc MSc PhD, Haider Sabah Kadhim MBChB, MSc, PhD and Wafa Mahmoud Al-Khalidy MBChB, DGO

ABSTRACT

HPV detection and genotyping from formalin-fixed, paraffinembedded (FFPE) tissues by using molecular analysis has become of great interest in recent years. The subjects included in this part of study were represented as formalin - fixed paraffin - embedded (FFPE) tissue blocks. The collection of tissue blocks of this study was carried out during the period from September 2013 to March 2014. A total of (100) samples were collected from the archives of histopathology department / Teaching Laboratories / Baghdad Medical City with different age ranged from (60 years old). Using a microtome serial tissue sections were cut into (3-10 μm) thickness, from each tissue block. Total DNA was extracted from the replicate tubes by two different methods, based on the DNeasy blood and tissue kit (Qiagen, Valencia, CA). The extraction done with two methods the first by paraffin removal by xylene and the other was processed by using a high-heat treatment. The prevalence of infection was 70% in FFPE samples, most of them are mixed infections (84.3%) and (15.7%) were single infections by types 16 (97.1%) and 18(72.9%) respectively. Of the total number 70/100 (70%) samples were positive in genotyping test. Recent finding showed that HPV 16 are responsible for (97.1) % of cases followed by HPV18 with (72.9%), HPV 31( 45.7%), HPV 45 (11.4%), HPV 59 (8.6%), HPV 51 (4.3%), HPV 35 (2.9%), HPV 36 (1.6%) respectively. The result of HPV typing showed that most of them were mixed infections by more than one type with majority to type 16 as it founded in 68 (97%) of samples . Of 70 samples that were PCR positive followed by type 18 (51) (72.9%).

Keywords: Human Papilloma Virus, Real Time PCR typing, DNA extraction.


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