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*Rubina A. Sattar Shertate and Prakash Ramrao Thorat


Reactive Red 81 was selected as model textile dye. The bacteriall strain Marinobacter santoriniensis DR-11 isolated from natural marine environment was screened for decolourization of Reactive Red 81. Optimization was carried out with respect to incubation conditions, carbon source, nitrogen source, pH and temperature. It was found that the optimum pH and temperature were 7.0% and 370C respectively. Glucose and yeast extract at 1.0 % concentration was found to give maximum decolourization. Further, it was found that the culture has brought about almost 98.00% decolourization of a recalcitrant dye, Reactive Red 81(3000μg/ml) at shaking condition within 24 hours. The present isolate also showed the degradation as evidenced from the reduction in the term of COD mg/L to the extent of 88.00 % within 24 hours. The biodegradation was monitored by GC-MS analysis. This study revealed the enormous biodegradation abilities of indigenous marine bacterial flora. The toxicity of the degraded products was checked by the microbial toxicity and fish toxicity and was revealed the degradation of Reactive Red 81 into non-toxic product by Marinobacter santoriniensis DR-11.

Keywords: Reactive Red 81, Bioremediation, GC-MS, Microbial Toxicity, Fish Toxicity.

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