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Abstract

QUANTIFICATION ASSAY OF METHOXSALEN FROM BULK DOSAGE FORM AND IT’S ALKALINE STRESS CONDITION BY UVSPECTROMETRY METHOD

Manisha B. Parhad*, Vishal R. Devhate and Koshish B. Gabhane

ABSTRACT

Rapid, specific and economic UV spectrophotometric method has been
developed to determine the methoxsalen content in bulk and
pharmaceutical dosage formulations. At a pre-determined λ max of
247nm, it was proved linear in the range of 1.0–12.0 mg/mL and
exhibited good correlation coefficient (R2=0.996). This method was
successfully applied to the determination of methoxsalen content in
marketed brand(Melanocyl R2-0.982, Meladerm R2-0.971) and the
results were in good agreement with the label claims. Methoxsalen is a
photosensitizing agent which has a chemical structure susceptible to
degradation. The obtained results proved that the method can be
employed for the routine analysis of methoxsalen in bulks as well as in
the commercial formulations.

Keywords: Methoxsalen, UV-Spectrscopy and Marketed preparation, Alkaline stress condition.


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