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Abstract

GAS CHROMATOGRAPHY AND MASS SPECTROSCOPY STUDIES ON LEAVES OF FICUS HISPIDA L.

Dhawale P. G.* and Ghyare B. P.

ABSTRACT

Objectives: To study the phytochemical constituents of leaves of Ficus hispida L. using Gas Chromatography and Mass Spectroscopic technique. Methods: The shade-dried leaves of F. hispida were extracted with methanol and acetone, the concentrated extracts were further subjected to GC-MS. Results: The GC-MS analyses determined the presence of 12 different phytochemical compounds in the methanol and acetone leaves extract of F. hispida. The phytoconsituents compounds were found in the mass spectra was matched with the National Institute of Standards and Technology (NIST) library. The major phytoconstituentsin methanol extract of leaves observed the presence of Octadecane,6-methyl- (0.67%), 2-Cis- 9-octadecenyloxyethanol (4.82%), Pentadecanoic acid,14-methyl-, methyl ester (0.84%), Estra-1,3,5 (10)-trien-17-ol (2.79%), Linolenic acid, methyl ester (1.18%), Cholestan-3-ol,2-methylene- (2.37%), Linolenin,1-mono (6.09%), Lonosta-8,24- dien-3-ol,acetate (6.77%), Squalene (5.08%), Stigmasterol (4.82%), Lup-20(29)-en-3-olacetate (39.11%) , β-Sitosterol (25.40%). Acetone extract of leaves observed the presence of Styrene (0.79%), 2-cis-9-octadecanyloxy ethanol (0.89%), Phen-1,4-diol,2,3-dimethyl-5- trifluromethyl (0.59%), Estra-1,3,5(10)-trien-17-ol (0.79%), Cholestan-3-ol-2-methylene (0.79%), Betulin (1.98%),1-Heptatricotanol (3.96%), 12-Olenen-3-yl-acetate (11.40%), Lonosta-8,24,dien-3-ol, acetate (11.90%), Squalene (4.96%), Lup-20(29)-en-3-ol-acetate (59.52%), β-Sitosterol (2.38%).Conclusions: This is the first report of documentation of active constituents from leaves of F. hispida. The results of the present study reveal that the leaves of F. hispida having effective potential bioactive compounds, which may be leads to the formulation of new drugs to treat various diseases.

Keywords: Ficus hispida, phytomedicines, phytoconstituents, GC-MS, secondary metabolites.


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