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Abstract

ASSOCIATION OF FSHR GENE POLYMORPHISM WITH POLYCYSTIC OVARY SYNDROME IN IRAQI WOMEN

Safa K. AL Salmani*, Norrya A. Ali and Wisam H. Salo

ABSTRACT

This study aims to detect the relationship between FSHR gene polymorphism and Polycystic Ovary Syndrome in Iraqi women using polymerase chain reaction –restriction fragment length polymorphism (PCR-RFLP). Fifty blood samples from Iraqi women with polycystic ovary syndrome (age ranged 16-45years) and twenty five blood samples from healthy women were collected from Kamal Al-Samarrai Hospital/ Baghdad, during the period from October 2014 to May 2015. DNA extraction using Bionear kit and the fragments of FSHR gene that contains the SNP rs6165 were amplified by PCR and then products were analyzed by RFLP using restriction enzyme AhdI. The new SNP rs6165 on FSHR shows no significant differences between patients and control group, which indicate the highly conservative sequence in Iraqi women. This is the first study to examine the association of FSHR gene polymorphism in an Iraqi ethnic to the best of the research knowledge.

Keywords: Polycystic Ovary Syndrome - FSHR gene polymorphism - PCR-RFLP.


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