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Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF CEFPODOXIME PROXETIL AND LEVOFLOXACIN HEMIHYDRATE IN COMBINED DOSAGE FORM

Supriya Ulhas Kubal* and Sachi S. Kudchadkar

ABSTRACT

A new UV spectrophotometric simultaneous equation method was developed and validated for the estimation of Cefpodoxime Proxetil (cefpo) Levofloxacin Hemihydrate (levo) in pure and pharmaceutical dosage form. Cefpo shows λmax at 235nm and levo shows at λmax 298nm in ethanol. The developed method was found to show linearity in concentration (conc.) range of 1-8 μg/ml for cefpo and levo with the value of correlation coefficient (R2) 0.999 and 0.996 respectively. The percent Relative Standard Deviation (% RSD) of inter and intra-day precision studies were found to be within limits of NMT 2% concluding that the present method is precise as per ICH guidelines Q2 (R1).[1] The developed method can be used for routine estimation of cefpo and levo in bulk and pharmaceutical dosage form.

Keywords: UV spectrophotometry, Cefpodoxime Proxetil, Levofloxacin Hemihydrate, Absorbance.


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