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Abstract

ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCRENNING OF EUPHORBIA LATHYRIS SEEDS

Wafaa Hamza Salman* and Mona AbdELMoniem

ABSTRACT

In this study we have carried out antimicrobial investigation and phytochemical analysis of Euphorbia lathyris seeds (Euphorbiaceae), Euphorbia lathyris seeds was extracted with 70% ethanol and then was fractionated with different solvents according to polarity start with petroleum ether, chloroform and ethanol, antimicrobial investigation was done for crude extract and all three fractions using cup plate diffusion method by selected six pathogens, four bacterias (Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa) and two fungi (Candida albicans and Aspergilles niger), crude extract and all fractions show antibacterial and antifungal activity aganist all bacterial and fungal, minimum inhibitory concentration (MIC) was done for crude and fractions compared with two antibiotics gentamycine and ciprofloxacin and also antifungal ketoconazole as standards, activity clear in first and second dilution for crude and fractions,more inhibition zone clear in bacillus .Phytochemical screening is an important step for the isolation of bioactive chemical constituents, phytochemicalScreeningwas done for crude extracts, which was extracted with 70% Ethanol was showed the presence of alkaloids, phytosterols, carbohydrates, flavanoides, coumarine and diterpenes.

Keywords: Antimicrobial, medicinal plants, Euphorbia lathyris, MIC, phytochemical screening.


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