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Abstract

ANTIMICROBIAL ACTIVITY AGAINST GANGRENE USING SILVER NANOPARTICLES SYNTHESIZED FROM ANDROGRAPHIS PANICULATA

J. Shanmuga Priya, *C. Priyadarshini, K. Poornima, Dr. S. Yamini Sudha Lakshmi, J. Madhusudhanan

ABSTRACT

Nanoparticles have been widely used in the field of nanoscience and medicine. In the present investigation, we synthesized nanoparticles using medicinally important plant Andrographis paniculata with 1mM and 3mM silver nitrate (AgNO3) solution in the ratio 1:9 (Leaf: Silver nitrate Solution) were used to synthesize the silver nanoparticle. They act as reducing agent. To synthesis the silver nanoparticle. The synthesized silver nanoparticles were characterized using UV-VIS spectroscopy, Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM) and Fourier Transform Infra-red Spectroscopy (FTIR). The appearance of dark brown colour and the UV absorbance for Andrographis paniculata at 430 nm confirms the synthesis of silver nanoparticles. The aim of the study is to determine the antibacterial and antifungal activity of the synthesized silver nanoparticles from the leaf extract of Andrographis paniculata. The most predominantly found microbial species in gangrene are Pseudomonas auringenosa, Escherichia coli, Klebsiella pneumoniae, Clostridium perferingens, Enterococcus faecalis, Candida albicans, Staphyllo coccus. The important outcome of the study will be the development of value added products from above mentioned medicinal plants of India for biological and nanotechnology based industries.

Keywords: Andrographis paniculata, Clostridium perferingens, Candida albicans, silver nanoparticle, Gangrene.


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