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Abstract

PHYSICO-CHEMICAL STANDARDISATION AND DEVELOPMENT OF HPTLC METHOD FOR THE DETERMINATION OF PLUMBAGIN IN CHITRAK HARITAKI – AN AYURVEDIC FORMULATION

Shivani, Iram Rakhshi, *Pawar R.K., Sharma Rajeev Kr. And Singh K.C.

ABSTRACT

A simple, rapid, selective and quantitative HPTLC method has been developed for determination of plumbagin in Ayurvedic formulations of Chitrak Haritaki of different manufactures. The chloroform extract of Chitrak Haritaki and Chitrak root samples were applied on TLC Aluminium plate pre coated with Silicagel60 GF254 and developed using Toluene : Ethyl acetate (3:1) v/v as a mobile phase. The plate was sprayed (derivatized) with Anisaldehyde‐Sulphuric Acid reagent followed by heating at 1100C for 10 minutes and detection and quantification were carried out densitometrically using an UV detector at wavelength of 270nm.

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