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*Tara Shankar Basuri, Rahul Solanki, Vishal S. Modi, Parixit Parajapati


High speed counter current chromatography (HSCCC) is a technique in which liquid stationary phase is use instead of solid supporting medium. HSCCC is a technique which provides number of advantages over other chromatographic techniques. In this article we focus on the principle, instrumentation and various application based on pharmaceutical science. In this HSCCC technique centrifugal partition technology is used to hold the liquid stationary phase in the column, while the liquid mobile phase is pushed through it that provides high purity and yield. The mechanism of HSCCC is based on the selection of two phase solvent system, partition coefficient, separation column, various solvent system, flow rate of mobile phase and revolution speed. In last few years great progress has been made in the pharmaceutical application of the HSCCC including extraction of medicinal drugs from plants and purification and isolation of active material, purification of dyes and compounds, plant analysis, rare earth elements‟ separation, analysis of inorganic compounds and elements, drug discovery and development.

Keywords: High Speed Counter Current Chromatography (HSCCC), centrifugal force, solvent system, application.

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