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Abstract

SPECTROPHOTOMETRIC DETERMINATION OF EMBELIN IN BULK AND PHARMACEUTICAL FORMULATION BY FIRST ORDER DERIVATIVE METHOD

Amey P. Nijsure*, Sugandha V. Mulgund, Shwetal P. Vassa

ABSTRACT

The aim of present work was to develop an accurate, precise, reproducible and economical First order derivative Spectrophotometric method for estimation of Embelin. The Embelin standard solution was scanned in UV range of 400-200nm in 1cm quartz cell in double beam spectrophotometer. The absorbance at min=282.1nm, max=320.8nm and zero cross=310.40nm was measured. The standard and sample solutions of embelin were prepared in methanol. The accuracy and precision of the method was determined and validated statistically. The method shows good recovery and reproducibility with % RSD less than 2. The method was found to be rapid, specific, precise and accurate and hence can be employed for routine analysis of embelin in bulk and its pharmaceutical dosage form.

Keywords: first order derivative, Embelin, UV Spectrophotometry, Quantitative estimation.


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