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Abstract

HPTLC ANALYSIS OF DOLICHANDRONE ATROVIRENS (SPRAGUE) PLANT BARK

P. Deepa*, S. Murugesh, R. Arivukkarasu

ABSTRACT

Dolichandrone atrovirens Sprague is a deciduous tree, It is belongs to the family Bignoniaceae. In this prospective study to evaluate the chromatogram detection of methanol extract of Dolichandrone atrovirens plant bark with standard flavonoid markers such as Apigenin, Rutin, Quercetin, Gallic acid and Catachin by HPTLC techniques. HPTLC Chromatogram was developed in methanolic extract Dolichandrone atrovirens plant bark and standard flavonoid marker compounds by using Toluene- Ethyl acetate- Formic acid- Methanol (3 : 6 : 1.6: 0.4) as mobile phase or solvent system. The identity of the bands of compounds 1-7 in the methanol extracts of Dolichandrone atrovirens were confirmed by overlaying their UV absorption spectra with the standard marker apigenin, rutin, quercetin, galic acid and catechin at 366 nm.

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