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Dr.Nihad Khalawe Tektook*, Eptissam Younan Pirko*
Dr. Hayat Kadhim Salman*


coagulase negative staphylococci (CONS) are now well established as major nosocomial pathogens associated with infections of indwelling medical devices, a total of 645 clinical samples, taken from different sources include blood, catheter urine specimen, wound and burn swabs and swabs of skin and nasal hospital staff, these sample had been cultured then isolated and identified pure isolates of different genus of bacteria, results show 85 (19.9%) were Pseudomonas spp isolates, 73 (17%) belong to Coagulase Negative Staphylococci (CONS) (S. epidermidis, S. saprophyticus, S.Lentus, S. haemolyticus and S.hominis constituted 68.5%, 13.7%, 6.85%, 6.85% and 4.1% Respectively), followed by both S. aureus and E. coli (n = 70,16.4%), followed by Klebsiella sp. (n = 68, 16%), Proteus sp. (n = 55,12.9%), Serratia marcescens (n = 6, 1.4%) isolated from study clinical samples. Second most important microorganism which isolated were Coagulase negative Staphylococci (n = 73, 17%), Staphylococcus epidermidis was mainly isolated from study patients group (68.5%) All isolates of CONS species are positive gram stain, produce white pigment except S.saprophyticus which produced yellow pigment and all isolates gave negative result of oxidase test, coagulase and catalase test was performed for all the isolates and all of them produced catalase enzyme that differentiates Staphylococcus from the genus Streptococcus, so Staphylococcus spp. reduce nitrate to nitrite and all isolates were identified by using epi staph and VITEK-2 system within 6 h., CONS are novobiocin resistance except S.saprophyticus. staph.epidermidis isolated were none fermented xylose, sorbitol, melibiose while fermented many sugar as positive results for glucose, fructose, sucrose, maltose, lactose and mannose, in addition to produce urease, lipase and alkaline phosphatase while it cannot produce DNase and lecithinase.

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