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Abstract

MICE EPIDIDYMAL SPERMS ACTIVATION IN VITRO BY MALE FERTILITY BLEND® MEDIUM: MODEL FOR MAMMALS

Al-Dujaily S.S.*, Farhan S.R. and Abbod M.S.

ABSTRACT

Background: Male Fertility Blend® (MFB) new nutrient supplements have different components especially Dong qui extract which may facilitate the sperm motility, quality and sperm count. However, in our knowledge there is no research concern on the effect of this new nutrient supplements on in vitro activation of epididymal sperms. Objective: This study was designed to study the effect of male fertility formula on the in vitro activated mice epididymal sperm parameters. Materials and Methods: In this study, MFB (0.1 mg/ml and 0.15 mg/ml culture medium) was used for in vitro direct sperm activation technique. Results: This study showed a significant (p<0.01) higher improvement in sperm characters by using 0.15% MFB than that of 0.1% MFB medium. The percentage of active sperm motility grade A using medium containing 0.15%MFB was highly significantly (P<0.01) increase compared to medium containing 0.1% MFB and medium free MFB. Conclusion: According to the results of present study, the investigation showed that the MFB medium contain many factors and energy sources with epididymal sperm of mice. This result can be utilized for IVF program in mammals.

Keywords: Male Fertility Blend®, epididymal sperm, in vitro activation.


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