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Abstract

EFFECT OF FEMALE FERTILITY BLEND® MEDIUM ON OOCYTE QUALITY AND MATURATION STATUS

Al-Dujaily S.S.*, Ismail A. and Abbod M.S.

ABSTRACT

Background: Female Fertility Blend® is a nutritional supplement for improving fertility in women containing vitamins, minerals, enzymes, amino acids, all may sustain the oocyte quality and ovarian function, at the same time protect oocyte from free radicals damage. Objective: The aim of the study is to examine the in vitro effect of Female Fertility Blend® (FFB) addition on oocyte maturation and to confirm the effect of Female Fertility Blend® on oocyte quality. Materials and Methods: In this study, 0.1ml from FFB concentrations (0.1, 0.15 mg/ml culture medium) was added to 7 ml of Ham_F12 medium for in vitro maturation, where the mature oocytes divided into three groups. The first group the gametes were cultured in Ham_F12 medium alone (FFB-free medium) and considered as control, second group the ova were cultured in0.1 mg/ml FFB- Ham_F12 culture medium, and the third group treated with 0.15 mg/ml FFB- Ham_F12 culture medium. Results: This study showed high significant (P<0.001) differences in maturation rate in both concentration of FFB that added to the medium (0.1, 0.15 mg/ml culture medium). Conclusions: It was concluded that the culturing and incubation of oocytes in a medium containing Female Fertility Blend® has a great improvement in oocyte maturation.

Keywords: Female Fertility Blend® medium, oocyte maturation, Hams-F12.


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