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Gajendra Singh Pushkar Choudhary Sajad Ahmad Mir Rajveer Singh Rawat, Dr. Bhanwar Lal Jat*


Plant based remedies have always been an integral part of traditional medicine throughout the world. The increasing demand for herbal medicines in the recent years due to fewer side effects in comparison to synthetic drugs and antibiotics has high lightened the need for the conservation and propagation of medicinal plants and there in vitro conservation of germplasm. The present study showed that it was possible to explore the morphogenetic potential of Lawscnia inermis by application of growth regulators and light condition. With the use of different combinations of cytokinins, the induction and multiplication of shoots derived from especial and Axillary meristems. The regulatory action of cytokinins and the apical dominance. The maximum shoot induction and multiplication was observed MS medium supplemented with 0.5mg/lit BA, 0.5 mg/l KN within 4 weeks of culture under a 14 hr photoperiod. At higher concentrations of BA or KN, the rate of shoot proliferation declined. The Axillary meristems produced more number of shoots than the apical meristems. Similar results were reported in Psoralea corylifolia. The results are consistent with earlier reports indicating that cytokinins and auxins affect shoot multiplication in other plants using shoot tip or Axillary bud explants. The results showed that the number of shoots per culture was increased in continuous light both in apical and Axillary meristems than in the 14 hr photoperiod. The interaction of photoperiod and plant growth regulators had a significant effect on shoot morphogenesis as reported earlier. With the increase in the concentration of either BA or KN, the percentage of shoot multiplication declined. The results also implied that there were differences among the treatments for both the percentage of culture developing multiple shoots and the mean numbers of shoots per culture. The variation of response was due to the varying concentration of growth regulators used in the medium and light condition as reported earlier in Lavandula latifolia and Zingiber officinal. The rate of multiplication was high and stable up to the 5th subculture and declined in subsequent subcultures. This might be due to the balancing of the endogenous and exogenous growth regulators and the ionic concentration of nutrient salts as reported earlier in other plants. The elongated shoots were rooted the maximum in MS basal salts supplemented with 0.25mg/Lit IBA with 2% sucrose. The rooting ability was reduced with the increase in the concentration of IAA or IBA in the medium. The percentage of shoots forming roots and days to rooting significantly varied with different concertinos of IAA or IBA.

Keywords: IAA, IBA, Lawscnia inermis (Henna), PGR, MS, In vitro.

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