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Abstract

ESTIMATION AND VALIDATION OF MONTELUKAST AND BAMBUTEROL BY CHROMATOGRAPHIC TECHNIQUES

Jayesh S. Patel* and Sarang D. Kulkarni

ABSTRACT

A simple, specific and accurate reverse phase high performance liquid chromatographic method for the estimation of Bambuterol and Montelukast in pharmaceutical dosage form. The HPLC system consisted of Younglin (S.K.) isocratic system UV detector. Model no. Acme900. The software used was Autochro – 3000. The column used in this method C8 (AGILENT). The configuration of the column is 4.6 x 250mm, particle size 5m., with mobile phase containing Methanol: Water (0.1%Triethylamine (TEA) pH adjusted to 3.3 with dil. Orthophosphoric(OPA) acid solution. The flow rate 0.7ml/min and effluents were monitores at 225nm. The recoveries of Bambuterol and Montelukast were found to be 98.85% to 102.25% w/v and 98.08% to 102.70% w/v, respectively. The proposed method was validate and successfully applied to the estimation of Bambuterol and Montelukast in combined tablet dosage forms.

Keywords: HPLC, Validation, Bambuterol, Montelukast.


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