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Abstract

ISOLATION AND MOLECULAR IDENTIFICATION OF AQUATIC BACTERIA FROM TIGRIS RIVER WITHIN WASIT PROVINCE

Helal H. Hays*, Hameed M. Jasim and Reyad A. Abduljabar

ABSTRACT

Water is essential to life, but many people do not have access to clean and safe drinking water and many die of waterborne bacterial infections. River water quality was affected by rainfall, sewage and miscellaneous human activities. In this study, water samples were collected from different locations of Tigris river stream within Wasit province from summer 2015 until spring 2016 for isolation and molecular identification of different bacterial strains. Water samples were taken from each place and kept in sterilized containers, then transported to laboratory under aseptic conditions. Aliquots of water samples were spread on nutrient agar plates and incubated at 28°C for 24 hours. Genomic DNA of pure single colonies grown on the medium was extracted for molecular identification of these isolates according to the nucleotide sequence of 16S rDNA gene. Results of extraction showed that pure DNA (250 μg/ml) was obtained and appear in single band after electrophoresis on agarose gel (1%). In order to amplify nucleotide sequence of 16S rDNA gene for each bacterial isolate, genomic DNA was first extracted from these isolates and amplified by using universal primers. The resulting amplified fragments were sequenced to determine the nucleotide sequence of 16S rDNA gene, then these sequences were aligned with related sequences for the same genes outlined in NCBI database. Results of molecular identification showed that these isolates are Bacillus subtilis, Escherichia coli, Bacillus sp., Exiguobacterium profundum, Enterobacter hormaechei, Pseudomonas compositi, Aeromonas veronii, Pseudomonas sp, Aeromonas caviae, Aeromonas hydrophila, Aeromonas punctata, Enterobacter cloacae and Aeromonas caviae.

Keywords: gene, 16S rDNA, the PCR technique, Tigris river, Wasit.


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