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Abstract

IN VITRO ANTIMICROBIAL ACTIVITY OF OLEANOLIC ACID IDENTIFIED IN CHLOROFORM EXTRACT OF FRUITS OF HELICTERES ISORA L.

Dinesh Kumar*, R. K. Singh and S. Farooq

ABSTRACT

The present study investigates the isolation and characterisation of Oleanolic acids from chloroform extract of fruits of Helicteres isora Linn and aim to prove Oleanolic acid as a antimicrobial agents that can be develop a new chemotherapy as antibiotics. Helicteres isora L is belongs to family Sterculiacae is a sub-deciduous shrub or small tree. It occurs, throughout India. The fruits are ground and used in colic pain, gripping bowels and flatulence. The fruits are astringent, acrid, refrigerant, demulcent, constipating, stomachic, vulnerary, haemostatic and urinary astringent. After chromatographic separation of compound HFCA, Antimicrobial activity have been performed and then chemical examination of HFCA done by IR,NMR techniques and finally it is proved that HFCA as a Oleanolic acid and zone of inhibition against S.aureus, E.coli, P.aeruginosa, B.cereus and A.flavus determines that Oleanolic acid acts as antimicrobial agents.

Keywords: Helicteres isora L, Antimicrobial activity. IR/UV,NMR Oleanolic acids.


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