Abstract

DETERMINATION OF SECONDARY METABOLITES AND ANTIOXIDANT ACTIVITY OF BRASSICA OLERACEA CAPITATA

V. Ramamurthy*, K. Durgadevi1, H. V. Anil Kumar and S. Raveendran

ABSTRACT

To quantify the major secondary metabolites and the antioxidant potential of ethanolic extract of Brassica oleracea. The ethanolic leaves plant extract of Brassica oleracea was analaysed by HPLC and GC to determine various Phytochemicals. Free radicals scavenging activity of extract by using DPPH, NO and Super oxide radicals generated in vitro. The ethanolic extract of B. oleracea was found to contain alkaloids, terpenoids, phenols and flavonoids. The major flavonoid detected was quercetin and rutin. The B. oleracea was found to possess significant radical scavenging activity against DPPH, NO and superoxide anions the IC50 value of 52.0 μg/ml, 52.0μg/ml and 52.6μg/ml respectively and comparable to that of their corresponding IC50 value. The medicinal property of B. oleracea may be attributed to the presence of flavonoids and phenolic compounds with rich antioxidant potential. The therapeutic effect of this plant may be accounted for its counteracting action on free radicals in vivo.

Keywords: Brassica oleracea, Phytochemicals, free radical, scavenging activity.