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Abstract

INVIVO TRACKING OF BMSCs LABELLED WITH (Gd-DTPA) BY MAGNETIC RESONANCE IMAGING IN MUSCULOSKELETAL TRAUMA

Perumal Saraswathi, Balaji Karuppaiah*, Nirmal Kumar. K, Kalyanapanchakshari.
P4, Saravana Kumar. S, Amarnath.C

ABSTRACT

In-vivo tracking of Bone marrow mesenchymal stem cells (BMSCs)
labeled with Gd-DTPA by magnetic resonance imaging serves as a
clinical road map in treating of musculoskeletal injuries. Once BMSCs
progress is analyzed inside the body, it can be used as a giant tool in
treating various diseases patterns. In this study, 1 Wistar rats and 6
New Zealand white rabbits, weighing 130-150gms and 2 - 2.5kgs were
used. BMSCs were labeled with gadolinium diethylene triamine Pentaacetic
acid (Gd-DTPA) and injected intraperitoneally into
musculoskeletal traumatized (MST) animals. After transplantation,
migrations of cells to the site of injury is tracked by magnetic
resonance imaging (MRI). After 4hrs of injection, BMSC labeled Gd-
DTPA cells observed with low signal intensity at the injured site.

Keywords: (Gd-DTPA) gadolinium diethylene triamine Penta-acetic acid, (MRI) Magnetic Resonance Imaging, (BMSCs) Bone marrow mesenchymal stem cells.


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