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Garima Pandey* and Sayyada Khatoon


Objective: Equisetum hyemale (EH) has been traditionally used for bone healing.
This study focussed on chemical profile and antioxidant potential of aerial part of EH
with a view to support its role in improving bone health. Methods: The
phytochemicals were identified and quantified using HPTLC. The analysis of macro
and micro elements was done using inductively coupled plasma mass spectrometer.
The antioxidant activity was studied using DPPH free radical scavenging, β-
Carotene-linoleic acid assay and reducing power assay. Results: The HPTLC studies
of aqueous-ethanol (50%) extract of EH (AEEH) showed the presence of rutin (429.4
μg. g-1), β-sitosterol (372.9 μg. g-1), lupeol (259.4 μg. g-1), diosgenin (163.1 μg. g-1),
and ellagic acid (27.5 μg. g-1). The key mineral elements identified in the dried
powder of EH were iron, zinc, manganese, copper and chromium. The total phenol
and total flavonoid content in AEEH were 332.32 mg.g-1 and 38.46 mg.g-1 dry
extracts, respectively. The AEEH showed significant (p<0.05) scavenging of DPPH
free radicals, IC 50 was 0.081 mg.ml-1. ). The anti-lipid peroxidation activity was
48.06% and 60.82 % at a concentration of 1.20 mg/ml for AEEH and α-tocopherol
respectively. The reducing power was recorded as 0.268 ± 0.045 for AEEH and 0.362 ± 0.043 for the standard
BHA, at a concentration of 1.25 mg/ml. Conclusion: Our study justified a prospective role of the E. hyemale in
bone healing, owing to the presence of various phytocompounds and significant antioxidant potential.

Keywords: Equisetum hyemale, Rutin, Diosgenin, Antioxidant, Bone healing.

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