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A. K. Dixit and Naureen Shaba Khan*


Nanotechnology is a blazing and burning area of present day research due to the fabulous, fiery and terrific distinguishing properties of nanoparticles from their bulk counter parts. Nanoparticles are the key to this technology. They are called as the building block as they exhibit large surface to volume ratio from their heavier source. Due to this they are in great demand and a compulsive requirement in every sector of society. Channeling of Bio-nanotechnology through this broad area has offered a new path of Nano-particle synthesis. Following this stream green chemistry approach has brought a drastic change in present scenario because it offers a comparatively safer and productive output in terms of environmental condition and suitability of method execution, it offers an ecofriendly and energy saving way to get the NPs. Keeping in view the benefits of verdure synthesis, present fraction of research work comprises the phytofabrication of Ag NPs using Centella asiatica‟s leaf extract as reducing and capping agent. The synthesized Ag NPs were characterized by UV-Vis Spectroscopy that confirms the formation of metallic nanoparticles by conferring the peak at (λmax) ~426nm Further, Transmission Electron Microscopy (TEM) and Nanoparticle Tracking analysis (NTA) were conducted to speculate the shape and size of phytosynthesized Ag NPs. The particles were found to be polydispersed spherical in nature with size distribution around 40±19nm. Also, the stability of Ag NPs was determined by Zeta sizer analysis which reveals negative zeta potential denoting negative surface charge (- 14.2 mV). Nanoparticles were found to be highly stable over a considerable period of time, which can be attributed to capping layer derived from reducing agent used to synthesize them.

Keywords: Green synthesis, Ag NPs, Centella asiatica, Characterization.

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