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Abstract

COMPARISON OF RAPID SLIDE CULTURE AND CONVENTIONAL LJ METHOD FOR DETECTION OF TB FROM SUSPECTED CASES OF TUBERCULOSIS

*Amruta Patil and Pratibha Desai

ABSTRACT

Tuberculosis is one of the dangerous disease in the world and its spreading very fast. There is emergence Drug resistance TB (MDR, XDR and TDR) because of many reasons. Transmission of MDR and XDR will be curse to human being. The objective of this study is to compared the Rapid slide culture (RSC) method with the growth on Lowenstein Jensen(LJ) media and to know the sensitivity and specificity of the rapid slide culture method. Total 100 sample was collected from suspected pulmonary tuberculosis cases. All the sample were subjected to three test:1.Zeihl Neelsen Staining 2. Rapid slide culture 3. Culture on Lowenstein Jensen media and the result were compared. Of the total 100 suspected sputum samples which were tested by all three methods, 57 samples were found to be Smear positive, 64 were found to be positive by the RSC method, 68 samples were found to be positive by the LJ culture method. The sensitivity of RSC was 91.17% specificity was 93.75%. Diagnosis can be done within 1 week with RSC where as 3-4 weeks by Conventional culturing method. So this method can be used in routine and especially in patient who have symptom of TB and have Negative smear of acid fast bacteria.

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