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Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF ONDANSETRON IN BULK AND PHARMACUTICAL FORMULATION

G.V.Sudhakararao*, K.Sujana, T.Pedababu

ABSTRACT

To develop simple, economical, precise and less time consuming UV method for the estimation of Ondansetron in bulk and pharmaceutical formulations. The method is based on UV spectroscopic technique. Ondansetron shows the maximum absorbance at 310nm in absorption maxima method. Drug followed the linearity in the range of 4-24μg/ml for this method with correlation coefficient (r2) of 0.999. The results of analysis have been validated statistically and recovery studies confirmed the accuracy of the proposed method. The method was validated as per the International Conference on Harmonization (ICH) guidelines. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and sensitive.

Keywords: Ondansetron, HPLC, UV spectrophotometry, absorption maxima method


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