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Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR STRYCHNINE IN NUX VOMICA CONTAINING FORMULATIONS

Bhargav Patel and Rajashree Mashru*

ABSTRACT

A selective, simple and efficient method-Reverse Phase High - performance liquid chromatography with UV detector was developed for the determination of active alkaloid, namely strychnine in Homeopathic formulations. The RP- HPLC separation was carried out using a Bischoff-chrombudget C18 (250mm x 4.6mm x5μm) column with a mobile phase consisting of Acetonitrile: Phosphate buffer pH- 3.6 with 0.2%TEA and 0.02% W/V SLS(55:45) hence providing high efficiency, high resolution and excellent peak shape for the analyte standard. The method was validated over the range of 1-6 μg/ml for strychnine. Accuracy is determined by parallel Spiking method instead of % recovery method because of very low concentration of an analyte in the formulations that can not be detected and %RSD was 1.9844. Interday precisions and Intraday precisions were within range. With a simple and minor sample preparation procedure and short run-time (<3 min), the proposed method was applicable for the quantitative analysis of strychnine in various homeopathic formulations.

Keywords: Validation, RP-HPLC, Strychnine, Homeopathic formulations.


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