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Abstract

PHARMACOGNOSTICAL STUDY AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF SOPHORA INTERRUPTA BEDD

Angilicam Avinash*, Samudrala Sudheer, Ganipineni Sumanth, Gundala Vinod Kumar, R. Ramasubramania Raja, Dr. M. Sreenivasulu and G. Madan Mohan

ABSTRACT

Sophora interrupta Bedd, was a common woody perennial herb native to India which belongs to Leguminosae family. The present study deals about anti-bacterial activity of Sophora interrupta. The antibacterial activity of benzene extract of Sophora interrupta bedd, was carried out by disc diffusion technique. The root extract was subjected to preliminary phytochemical screening. The benzene extract of Sophora interrupta of different concentrations about 0.25, 0.5, 0.75, 1.0, 1.25,1.50mg/ml was made dissolved in DMSO. These solutions were sterilized using filtration sterilization technique, these dilutions were used to test the antibacterial activity of 3 different strains viz, Bacillus subtilis, Pseudomonas aureus and Pseudomonas syringe. The obtained results were compared with standard drug Ciprofloxacin. The minimum inhibitory concentration was determined for the concerned microorganism. From the experimental results it can be concluded that root extract of Sophora interrupta Bedd., has very good Antibacterial activity.

Keywords: Antibacterial activity, Benzene extract, Ciprofloxacin, Disc diffusion technique, Sophora interrupta.


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