WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

ASSESSMENT OF CYTOTOXIC EFFECT OF METHANOLIC EXTRACTS OF LEUCAS ASPERA AND TAGETES ERECTA USING CULTURED HUMAN MONONUCLEAR CELLS

Arif Nisha Syad*, Jeyashri M., Banu Priya S., Muthamizhselvi and Thirumalai Vasan P.

ABSTRACT

Natural products have been the most successful source of potential drug leads. Natural products have been used since ancient times and in folklore for the treatment of many diseases and illnesses. Peripheral Blood Mononuclear Cells (PBMC) was engaged for assessing the toxicity of various concentrations (100 – 500 μg/ml) of methanolic leaf extract of L. aspera and methanolic flower extract of T. erecta. Trypan blue exclusion assay was performed for different time peroid 24, 48 and 72 h. Results of the present study suggest that the lymphocytes when treated with (100 μg/ml and 200 μg/ml) L. aspera, the percentage of viability was 71.84 ± 2.91% and 66.66 ± 5.36% and 69.5 ± 0% and 66 ± 9.61% after incubating at 24 and 48 h respectively. In the case of T. erecta, percentage of cell viability upon treatment with 100 μg/ml and 200 μg/ml of methanolic extract at 24 and 48 h was found to be 90 ± 0% and 81.88 ± 0% (24 h) and 81.8 ± 0% and 81.2 ± 1.05% (48 h) respectively. Hemolysis assay was performed on red blood cells and cells treated with L. aspera extract (100 μg/ml and 200 μg/ml) did not show any increase in the absorbance at 540 nm. Whereas, cells treated with T. erecta at a 500 μg/ml exhibited only marginal increase in the absorbance value (0.246) when compared with 100 μg/ml (0.116). The above results suggest that the methanolic extract of did not exhibit toxic effects even at the concentration of 200 μg/ml.

Keywords: Leucas aspera, Tagetes erecta, Peripheral Blood Mononuclear Cells (PBMC), Tryphan blue, Cell viability, Menthanolic extract.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More