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Abstract

PHYTOCHEMICAL SCREENING, ANTIGLYCATION AND ANTIOXIDANT ACTIVITIES OF ETHANOLIC LEAF EXTRACT OF AZIMA TETRACANTHA LAM.

S. Josephinol*, T. Nargis Begum# and M.H. Muhammad [email protected]

ABSTRACT

Objective: Assessment of in vitro antioxidant and in vitro antidiabetic potential of Azima tetracantha Lam. Methods: Phytochemicals of hexane, aqueous and ethanolic extracts of the Azima tetracantha Lam were analyzed. The antioxidant potential of hexane, aqueous and Ethanolic extracts was assessed by 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing ability power (FRAP), superoxide (O2−), hydrogen peroxide (H2O2)and nitric oxide (NO) scavenging assay and antidiabetic activity was assessed by α-amylase and α-glucosidase inhibition. Results: Amongst all the three extracts of A. tetracantha, ethanol leaf extracts contain significant antioxidant as well as antidiabetic potential. A. tetracantha showed potential antioxidant as well as anti-diabetic activities and have good reputation in traditional Indian medicine. Conclusion: Ethanol leaf extracts of A. tetracanthais having high medicinal potential in terms of their antioxidant and antidiabetic activities. Studies are required to further elucidate antioxidant and anti-diabetic potentials using in-vivo biochemical and molecular biology techniques.

Keywords: Azima tetracantha; DPPH; ?-amylase inhibition; ?-glucosidase inhibition.


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